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human granzyme b elisa kit  (Elabscience Biotechnology)
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Elabscience Biotechnology human granzyme b elisa kit
Down-regulation of HMGB2 enhances the function of NK cells. (a) qRT-PCR analysis of HMGB2 mRNA levels in NK-92 cells transfected with siNC, siHMGB2 #1, siHMGB2 #2, and siHMGB2 #3. (b) Western blot analysis of HMGB2 protein levels in NK-92 cells transfected with siNC or siHMGB2. (c) The secretion levels of perforin-1 and granzyme B were measured by <t>ELISA</t> in NK-92 cells after HMGB2 knockdown. (d) Cytokine release (TNF-α and IFN-γ) from NK-92 cells with HMGB2 knock down were showed in histogram. (e) Cytotoxicity of NK-92 cells with HMGB2 knockdown against various ESCC cell lines (EC109, KYSE70, KYSE150, KYSE450) was assessed at 24h and 48h using a cytotoxicity assay. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test for normally distributed data, or Kruskal–Wallis tests followed by Dunn’s post hoc tests for non-normally distributed data. * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SD.
Human Granzyme B Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/elabscience+human+gzmb/Human+GzmB+(Granzyme+B)+ELISA+Kit/pmc12634629-93-20-26
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human granzyme b elisa kit - by Bioz Stars, 2026-09
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Down-regulation of HMGB2 enhances the function of NK cells. (a) qRT-PCR analysis of HMGB2 mRNA levels in NK-92 cells transfected with siNC, siHMGB2 #1, siHMGB2 #2, and siHMGB2 #3. (b) Western blot analysis of HMGB2 protein levels in NK-92 cells transfected with siNC or siHMGB2. (c) The secretion levels of perforin-1 and granzyme B were measured by ELISA in NK-92 cells after HMGB2 knockdown. (d) Cytokine release (TNF-α and IFN-γ) from NK-92 cells with HMGB2 knock down were showed in histogram. (e) Cytotoxicity of NK-92 cells with HMGB2 knockdown against various ESCC cell lines (EC109, KYSE70, KYSE150, KYSE450) was assessed at 24h and 48h using a cytotoxicity assay. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test for normally distributed data, or Kruskal–Wallis tests followed by Dunn’s post hoc tests for non-normally distributed data. * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SD.

Journal: Frontiers in Immunology

Article Title: NK cell function down regulated by HMGB2 through ANGPT1/PI3K/AKT pathway and its effect on esophageal squamous carcinoma cells

doi: 10.3389/fimmu.2025.1666199

Figure Lengend Snippet: Down-regulation of HMGB2 enhances the function of NK cells. (a) qRT-PCR analysis of HMGB2 mRNA levels in NK-92 cells transfected with siNC, siHMGB2 #1, siHMGB2 #2, and siHMGB2 #3. (b) Western blot analysis of HMGB2 protein levels in NK-92 cells transfected with siNC or siHMGB2. (c) The secretion levels of perforin-1 and granzyme B were measured by ELISA in NK-92 cells after HMGB2 knockdown. (d) Cytokine release (TNF-α and IFN-γ) from NK-92 cells with HMGB2 knock down were showed in histogram. (e) Cytotoxicity of NK-92 cells with HMGB2 knockdown against various ESCC cell lines (EC109, KYSE70, KYSE150, KYSE450) was assessed at 24h and 48h using a cytotoxicity assay. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test for normally distributed data, or Kruskal–Wallis tests followed by Dunn’s post hoc tests for non-normally distributed data. * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SD.

Article Snippet: After centrifugation, the culture supernatant was collected, and the concentrations of granzyme B and perforin 1 were measured using the Human Granzyme B ELISA Kit (E-EL-H1617c, Elabscience, Beijing, China) and Human Perforin 1 ELISA Kit (E-EL-H1123c, Elabscience), respectively, according to the manufacturer’s instructions.

Techniques: Quantitative RT-PCR, Transfection, Western Blot, Enzyme-linked Immunosorbent Assay, Knockdown, Cytotoxicity Assay

Overexpression of HMGB2 inhibits the function of NK-92 cells. (a) qRT-PCR analysis of HMGB2 mRNA levels in NK-92 cells transfected with pcDNA3.1-Vector or pcDNA3.1-HMGB2. (b) Western blot analysis of HMGB2 protein expression in NK-92 cells transfected with pcDNA3.1-Vector or pcDNA3.1-HMGB2. (c) The secretion levels of perforin-1 and granzyme B were measured by ELISA in NK-92 cells after HMGB2 overexpression. (d) Cytokine release (TNF-α and IFN-γ) from NK-92 cells with HMGB2 overexpression was showed in histogram. (e) Cytotoxicity of NK-92 cells with HMGB2 overexpression against various ESCC cell lines (EC109, KYSE70, KYSE150, KYSE450) at 24h and 48h was assessed using a cytotoxicity assay. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test for normally distributed data, or Kruskal–Wallis tests followed by Dunn’s post hoc tests for non-normally distributed data. * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SD.

Journal: Frontiers in Immunology

Article Title: NK cell function down regulated by HMGB2 through ANGPT1/PI3K/AKT pathway and its effect on esophageal squamous carcinoma cells

doi: 10.3389/fimmu.2025.1666199

Figure Lengend Snippet: Overexpression of HMGB2 inhibits the function of NK-92 cells. (a) qRT-PCR analysis of HMGB2 mRNA levels in NK-92 cells transfected with pcDNA3.1-Vector or pcDNA3.1-HMGB2. (b) Western blot analysis of HMGB2 protein expression in NK-92 cells transfected with pcDNA3.1-Vector or pcDNA3.1-HMGB2. (c) The secretion levels of perforin-1 and granzyme B were measured by ELISA in NK-92 cells after HMGB2 overexpression. (d) Cytokine release (TNF-α and IFN-γ) from NK-92 cells with HMGB2 overexpression was showed in histogram. (e) Cytotoxicity of NK-92 cells with HMGB2 overexpression against various ESCC cell lines (EC109, KYSE70, KYSE150, KYSE450) at 24h and 48h was assessed using a cytotoxicity assay. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test for normally distributed data, or Kruskal–Wallis tests followed by Dunn’s post hoc tests for non-normally distributed data. * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SD.

Article Snippet: After centrifugation, the culture supernatant was collected, and the concentrations of granzyme B and perforin 1 were measured using the Human Granzyme B ELISA Kit (E-EL-H1617c, Elabscience, Beijing, China) and Human Perforin 1 ELISA Kit (E-EL-H1123c, Elabscience), respectively, according to the manufacturer’s instructions.

Techniques: Over Expression, Quantitative RT-PCR, Transfection, Plasmid Preparation, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Cytotoxicity Assay

Confirmation of HMGB2 knockout and functional assessment of KO-HMGB2 NK-92 cells. (a) Sanger sequencing chromatograms showing two distinct allelic edits in the KO-HMGB2 NK-92 cells. (b) Western blot analysis of HMGB2 protein levels in wild-type (WT) and KO-HMGB2 NK-92 cells. (c) ELISA analysis of perforin-1 and granzyme B secretion in supernatants collected from KO-HMGB2 and WT NK-92 cells co-cultured with KYSE450 cells for 12 hours. (d) qRT-PCR analysis of activating receptors (NKG2D, NKp44, NKp46, and CD107a) in KO-HMGB2 and WT NK-92 cells. (e) Cytotoxicity of NK-92 cells with HMGB2 knock out against various ESCC cell lines (EC109, KYSE70, KYSE150, KYSE450) at 24h and 48h was assessed using a cytotoxicity assay. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test for normally distributed data, or Kruskal–Wallis tests followed by Dunn’s post hoc tests for non-normally distributed data. * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SD.

Journal: Frontiers in Immunology

Article Title: NK cell function down regulated by HMGB2 through ANGPT1/PI3K/AKT pathway and its effect on esophageal squamous carcinoma cells

doi: 10.3389/fimmu.2025.1666199

Figure Lengend Snippet: Confirmation of HMGB2 knockout and functional assessment of KO-HMGB2 NK-92 cells. (a) Sanger sequencing chromatograms showing two distinct allelic edits in the KO-HMGB2 NK-92 cells. (b) Western blot analysis of HMGB2 protein levels in wild-type (WT) and KO-HMGB2 NK-92 cells. (c) ELISA analysis of perforin-1 and granzyme B secretion in supernatants collected from KO-HMGB2 and WT NK-92 cells co-cultured with KYSE450 cells for 12 hours. (d) qRT-PCR analysis of activating receptors (NKG2D, NKp44, NKp46, and CD107a) in KO-HMGB2 and WT NK-92 cells. (e) Cytotoxicity of NK-92 cells with HMGB2 knock out against various ESCC cell lines (EC109, KYSE70, KYSE150, KYSE450) at 24h and 48h was assessed using a cytotoxicity assay. Statistical significance was determined using one-way ANOVA with Tukey’s post hoc test for normally distributed data, or Kruskal–Wallis tests followed by Dunn’s post hoc tests for non-normally distributed data. * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars represent the mean ± SD.

Article Snippet: After centrifugation, the culture supernatant was collected, and the concentrations of granzyme B and perforin 1 were measured using the Human Granzyme B ELISA Kit (E-EL-H1617c, Elabscience, Beijing, China) and Human Perforin 1 ELISA Kit (E-EL-H1123c, Elabscience), respectively, according to the manufacturer’s instructions.

Techniques: Knock-Out, Functional Assay, Sequencing, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture, Quantitative RT-PCR, Cytotoxicity Assay